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| Registros recuperados: 24 | |
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| Barbosa,Alexandre Naime; Boyer,Leslie; Chippaux,Jean-Philippe; Medolago,Natalia Bronzatto; Caramori,Carlos Antonio; Paixão,Ariane Gomes; Poli,João Paulo Vasconcelos; Mendes,Mônica Bannwart; Santos,Lucilene Delazari dos; Ferreira Jr,Rui Seabra; Barraviera,Benedito. |
| Abstract Background Envenomation caused by multiple stings from Africanized honeybees Apis mellifera constitutes a public health problem in the Americas. In 2015, the Brazilian Ministry of Health reported 13,597 accidents (incidence of seven cases per 100,000 inhabitants) with 39 deaths (lethality of 0.25%). The toxins present in the venom, which include melittin and phospholipase A2, cause lesions in diverse organs and systems that may be fatal. As there has been no specific treatment to date, management has been symptomatic and supportive only. Methods In order to evaluate the safety and neutralizing capacity of a new apilic antivenom, as well as to confirm its lowest effective dose, a clinical protocol was developed to be applied in a multicenter,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Apis mellifera; Bee venom; Toxins; Envenomation; Heterologous serum; Apilic antivenom; Bee antivenom. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100309 |
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| Mozafari,Roghayeh; Kyrylenko,Sergiy; Castro,Mateus Vidigal; Ferreira Jr,Rui Seabra; Barraviera,Benedito; Oliveira,Alexandre Leite Rodrigues. |
| Abstract Background Peripheral nerve injury is a worldwide clinical problem, and the preferred surgical method for treating it is the end-to-end neurorrhaphy. When it is not possible due to a large nerve gap, autologous nerve grafting is used. However, these surgical techniques result in nerve regeneration at highly variable degrees. It is thus very important to seek complementary techniques to improve motor and sensory recovery. One promising approach could be cell therapy. Transplantation therapy with human embryonic stem cells (hESCs) is appealing because these cells are pluripotent and can differentiate into specialized cell types and have self-renewal ability. Therefore, the main objective of this study was to find conditions under which functional... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Neurorrhaphy; Sciatic nerve; Human embryonic stem cells; Fibrin sealant; FGF2. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100305 |
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| Oliveira,Laudicéia Alves de; Ferreira Jr,Rui Seabra; Barraviera,Benedito; Carvalho,Francilene Capel Tavares de; Barros,Luciana Curtolo de; Santos,Lucilene Delazari dos; Pimenta,Daniel Carvalho. |
| Abstract Background Classically, Crotalus durissus terrificus (Cdt) venom can be described, according to chromatographic criteria, as a simple venom, composed of four major toxins, namely: gyroxin, crotamine, crotoxin and convulxin. Crotoxin is a non-covalent heterodimeric neurotoxin constituted of two subunits: an active phospholipase A2 and a chaperone protein, termed crotapotin. This molecule is composed of three peptide chains connected by seven disulfide bridges. Naturally occurring variants/isoforms of either crotoxin or crotapotin itself have already been reported. Methods The crude Cdt venom was separated by using RP-HPLC and the toxins were identified by mass spectrometry (MS). Crotapotin was purified, reduced and alkylated in order to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Crotalus durissus terrificus; Venom; Crotoxin; Crotapotin; Isoforms. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100319 |
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| Martinez,Melissa Gaste; Ducatti,Carlos; Silva,Evandro Tadeu; Sant’Anna,Savio Stefanini; Sartori,Maria Márcia Pereira; Barraviera,Benedito. |
| Background Environmental devastation threatens the survival of many species, including venomous snakes such as the South American rattlesnake Crotalus durissus terrificus. This observation is based on the decrease of snakes collected and donated to Brazilian research institutes. Nevertheless, some individuals have managed to survive and procreate. The question is how these snakes are adapting in these new environmental conditions.Methods To answer it, the carbon-13 level of rattlesnakes and their feed (either laboratory or wild mice) was evaluated by isotope-ratio mass spectrometry. Thus, rattle segments from 16 adults and 15 offspring of captive snakes, and of three wild newborn C. d. terrificus were evaluated as well as 17 Mus musculus mice captured in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Food; Carbon-13; Crotalus durissus terrificus; Stable isotopes. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200342 |
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| Cassaro,Claudia Vilalva; Justulin Jr.,Luis Antonio; Lima,Patrícia Rodrigues de; Golim,Marjorie de Assis; Biscola,Natália Perussi; Castro,Mateus Vidigal de; Oliveira,Alexandre Leite Rodrigues de; Doiche,Danuta Pulz; Pereira,Elenize Jamas; Ferreira Jr.,Rui Seabra; Barraviera,Benedito. |
| ABSTRACT Background: Bone tissue repair remains a challenge in tissue engineering. Currently, new materials are being applied and often integrated with live cells and biological scaffolds. The fibrin biopolymer (FBP) proposed in this study has hemostatic, sealant, adhesive, scaffolding and drug-delivery properties. The regenerative potential of an association of FBP, biphasic calcium phosphate (BCP) and mesenchymal stem cells (MSCs) was evaluated in defects of rat femurs. Methods: Adult male Wistar rats were submitted to a 5-mm defect in the femur. This was filled with the following materials and/or associations: BPC; FBP and BCP; FBP and MSCs; and BCP, FBP and MSCs. Bone defect without filling was defined as the control group. Thirty and sixty days... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bone regeneration; Biomaterials; Fibrin sealant; Fibrin biopolymer; Biphasic calcium phosphate; Mesenchymal stem cells. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100320 |
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| Ferreira Jr,Rui Seabra; Barros,Luciana Curtolo de; Abbade,Luciana Patrícia Fernandes; Barraviera,Silvia Regina Catharino Sartori; Silvares,Maria Regina Cavariani; Pontes,Leticia Gomes de; Santos,Lucilene Delazari dos; Barraviera,Benedito. |
| Abstract Hemostatic and adhesive agents date back to World War II, when homologous fibrin sealant came onto scene. Considering that infectious diseases can be transmitted via human blood, a new heterologous fibrin sealant was standardized in the 1990s. Its components were a serine protease (a thrombin-like enzyme) extracted from the venom of Crotalus durissus terrificus snakes and a fibrinogen-rich cryoprecipitate extracted from the blood of Bubalus bubalis buffaloes. This new bioproduct has been used as a coagulant, sealant, adhesive and recently as a candidate scaffold for mesenchymal stem cells and bone and cartilage repair. This review discusses the composition of a new heterologous fibrin sealant, and cites published articles related to its... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fibrin sealant; Snake venom; Cryoprecipitate coagulum; Thrombin-like enzyme; Buffaloes. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100204 |
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| Barros,Gustavo A. C.; Pereira,Andreia V.; Barros,Luciana C.; Lourenço Jr,Airton; Calvi,Sueli A.; Santos,Lucilene D.; Barraviera,Benedito; Ferreira Jr,Rui Seabra. |
| Abstract Background American visceral leishmaniasis is caused by the intracellular parasiteLeishmania (L.) infantum chagasi, and transmitted by the sand fly Lutzomyia longipalpis. Since treatment is based on classical chemotherapeutics with significant side effects, the search for new drugs remains the greatest global challenge. Thus, this in vitro study aimed to evaluate the leishmanicidal effect ofCrotalus durissus terrificus venom fractions on promastigote and amastigote forms of Leishmania (L.) infantum chagasi. Methods Phospholipase A 2 (PLA 2 ) and a pool of peptide fraction (<3 kDa) were purified from Crotalusvenom. Furthermore, promastigotes and peritoneal macrophages of mice infected by amastigotes were exposed to serial dilutions of the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: PLA2; Peptides; Crotalus dutissus terrificus; Venom; Leishmanicidal activity. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100351 |
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| Pereira,Andreia Vieira; Barros,Gustavo de; Pinto,Erika Gracielle; Tempone,Andre Gustavo; Orsi,Ricardo de Oliveira; Santos,Lucilene Delazari dos; Calvi,Sueli; Ferreira Jr,Rui Seabra; Pimenta,Daniel Carvalho; Barraviera,Benedito. |
| Abstract Background Apis mellifera venom, which has already been recommended as an alternative anti-inflammatory treatment, may be also considered an important source of candidate molecules for biotechnological and biomedical uses, such as the treatment of parasitic diseases. Methods Africanized honeybee venom from Apis mellifera was fractionated by RP-C18-HPLC and the obtained melittin was incubated with promastigotes and intracellular amastigotes of Leishmania (L.) infantum. Cytotoxicity to mice peritoneal macrophages was evaluated through mitochondrial oxidative activity. The production of anti- and pro-inflammatory cytokines, NO and H2O2 by macrophages was determined. Results Promastigotes and intracellular amastigotes were susceptible to melittin... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Melittin; Apis mellifera; Leishmania; Leishmaniasis; Peptides; Toxins; Antiparasitic; Cytokines. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100301 |
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| Biscola,Natalia Perussi; Cartarozzi,Luciana Politti; Ulian-Benitez,Suzana; Barbizan,Roberta; Castro,Mateus Vidigal; Spejo,Aline Barroso; Ferreira Jr.,Rui Seabra; Barraviera,Benedito; Oliveira,Alexandre Leite Rodrigues. |
| Abstract Lesions to the nervous system often produce hemorrhage and tissue loss that are difficult, if not impossible, to repair. Therefore, scar formation, inflammation and cavitation take place, expanding the lesion epicenter. This significantly worsens the patient conditions and impairment, increasing neuronal loss and glial reaction, which in turn further decreases the chances of a positive outcome. The possibility of using hemostatic substances that also function as a scaffold, such as the fibrin sealant, reduces surgical time and improve postoperative recovery. To date, several studies have demonstrated that human blood derived fibrin sealant produces positive effects in different interventions, becoming an efficient alternative to suturing. To... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Central nervous system; Peripheral nervous system; Commercial fibrin sealant; New heterologous fibrin sealant; Nervous system injury; Fibrin tissue adhesive. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100202 |
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| Chippaux,Jean-Philippe; Akaffou,Marc Hermann; Allali,Bernard Kouadio; Dosso,Mireille; Massougbodji,Achille; Barraviera,Benedito. |
| Abstract During the 6th International Conference on Envenomation by Snakebites and Scorpion Stings in Africa held in Abidjan, from 1 to 5 June 2015, the measures for the management of envenomation were discussed and new recommendations were adopted by the participants. The high incidence and severity of this affliction were confirmed by several studies conducted in African countries. The poor availability of antivenom, particularly because of the cost, was also highlighted. Some experiences have been reported, mainly those regarding the financial support of antivenom in Burkina Faso (more than 90 %) and Togo (up to 60 %) or the mandatory reporting of cases in Cameroon. Key recommendations concerned: improvement of epidemiological information based on case... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Envenomation; Antivenom; Epidemiology; Drug policy; Africa. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100101 |
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| Registros recuperados: 24 | |
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